Location: Aquatic Animal Health Research
Title: Differential Channel Catfish Ovary Cell Gene Expression after Experimental Infection with Edwardsiella Ictaluri Authors
Submitted to: American Society for Microbiology Annual Meeting
Publication Type: Proceedings
Publication Acceptance Date: March 15, 2006
Publication Date: May 1, 2006
Citation: Yeh, H., Klesius, P.H. 2006. Diferential channel catfish ovary cell gene expression after experimental infection with Edwardsiella ictaluri. 106th Annual Meeting of the American Society for Microbiology Orlando, FL. Abstract No. Z-005. Technical Abstract: Background: Enteric septicemia of catfish, caused by Edwardsiella ictaluri, is among the most common diseases of channel catfish (Ictalurus punctatus) and is responsible for $50 - 60 million economic losses to catfish producers annually in the Southeastern U.S. This study investigated gene expression in channel catfish ovary (CCO) cells after infection with E. ictaluri. Method: CCO cells were infected with E. ictaluri at 0.5 CFU per cell. After six hours post infection, cells were harvested, mRNAs were isolated and differential cDNA expression libraries were constructed by the suppression subtractive hybridization technique. DNA sequences were sequenced and analyzed by various bioinformatic computer programs. Tentative gene identification was based on an E value of less than 0.001. Results: A number of up-regulated gene transcripts were identified that are associated with expression of innate immunity, i.e. immunophilins, nitric oxide synthase, ubiquitin-proteasome system, zebrafish homologs of CD 147, CD81, CD59, CD63, and extracellular matrix (galectin-4, laminin receptor 1 and fibronectin 1a). In addition, transcripts of homologs to metabolic genes were demonstrated to be up-regulated. The up-regulation of selected gene transcripts were further validated by PCR and real-time PCR. Conclusion: The results of this study provide useful information on the molecular basis of E. ictaluri-induced gene expression in experimentally infected CCO cells and hopefully accelerate the understanding of innate mechanism of immunity.