ARS | Publication request: Channel catfish gene expression after Edwardsiella ictaluri infection.

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: March 10, 2006
Publication Date: July 25, 2006
Citation: Yeh, H., Klesius, P.H. 2006. Channel catfish gene expression after Edwardsiella ictaluri infection. Genbank.

Technical Abstract: Channel catfish Ictalurus punctatus infected with Edwardsiella ictaluri results in $50 - 60 million annual losses in profits to catfish producers. To explore channel catfish gene expression after Edwardsiella ictaluri, a subtractive-suppression hybridization method was used. The plasmid DNAs were sequenced from 5'-end using a T3 primer. Sequences were trimmed and aligned using Phred/Phrap/Consed programs. Trimmed sequences containing more than 100 bases were subject to BLAST against the GenBank databases. Top hits with a bit score greater than 100 and an E-value less than 0.001 were selected for further annotation. In this report, 500 sequences from forward and backward subtractions of infected channel catfish ovary cells were identified.