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United States Department of Agriculture

Agricultural Research Service

Title: Characterization of Salmonella Enterica Serovar Agona Slaughter Isolates from the Animal Arm of the National Antimicrobial Resistance Monitoring System – Enteric Bacteria (Narms): 1997 Through 2003

item Cray, Paula
item Douris, Aphrodite
item Jackson, Charlene
item Anandaraman, Neena - USDA-FSIS
item Rose, Bonnie - USDA-FSIS

Submitted to: Proceedings of the International Conference on Emerging Infectious Diseases
Publication Type: Abstract Only
Publication Acceptance Date: February 20, 2006
Publication Date: March 20, 2006
Citation: Cray, P.J., Douris, A., Jackson, C.R., Anandaraman, N., Rose, B. 2006. Characterization of salmonella enterica serovar agona slaughter isolates from the animal arm of the national antimicrobial resistance monitoring system – enteric bacteria (narms): 1997 through 2003 [abstract]. Proceedings of the International Conference on Emerging Infectious Diseases. 113:85.

Technical Abstract: Background: Currently, there is limited published data relative to the prevalence, antimicrobial susceptibility, and molecular subtypes of Salmonella enterica serovar Agona present in slaughter samples from cattle, chicken, turkey and swine in the US. The objectives of this study were to establish baseline information for S. Agona prevalence in food animal slaughter samples from 1997 through 2003. Methods: A total of 499 Salmonella enterica serovar Agona isolates were assayed for antimicrobial susceptibility and subtyped using pulsed field gel electrophoresis. All isolates were collected as part of the animal arm of the National Antimicrobial Resistance Monitoring System - Enteric Bacteria (NARMS) from slaughter and processing samples at federally inspected plants. Isolates originated from cattle, swine, chicken, and turkey samples for the years 1997 through 2003. Results: Salmonella Agona isolates exhibited increased resistance to six of the 19 antimicrobials tested: amoxicillin/clavulanic acid, ampicillin, cefoxitin, ceftiofur, cephalothin, and chloramphenicol. Although all isolates were susceptible to ciprofloxacin, an increase in resistance to the quinolone, nalidixic acid was observed. A single isolate was resistant to ceftriaxone; however, increased resistance to the other cephalosporins (cefoxitin, ceftiofur, and cephalothin) was observed. Multiple drug resistance (MDR; resistance >2 antimicrobials) was exhibited in 57% (n=282/499) of the S. Agona isolates and 22% (n=111/499) of these S. Agona isolates were resistant to five or more antimicrobials. A majority of the S. Agona isolates originated from cattle (40%; n=202/499) and represented 77% (n=85/111) of the MDR isolates resistant to five or more antimicrobials. Cluster analysis indicated that isolates did not group together based on year isolate was recovered, geographical region, or animal source. However, groupings that were indistinguishable by PFGE appeared to correspond with antimicrobial resistance profiles. Conclusions: These data suggest that S. Agona is increasing in prevalence in U.S. cattle present for slaughter and should be further monitored.

Last Modified: 5/5/2015