ARS | Publication request: GENOMIC ANALYSIS OF THE EARLY RESPONSES OF DEVELOPING RICE SEEDLINGS TO COLD STRESS

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 11, 2005
Publication Date: January 15, 2006
Citation: Cheng, C., Zhang, Y., Ressom, H., Jia, Y., Yun, S., De Los Reyes, B.G. 2006. Genomic analysis of the early responses of developing rice seedlings to cold stress [abstract]. American Society of Plant Biologists Annual Meeting. Available: www.aspb.org/search

Technical Abstract: The changes in the transcriptome of developing rice seedlings (cv. CT6748-8-CA-17) during exposure to low temperature (10oC) was investigated using a microarray containing more than 6,000 cDNAs from cold and drought stress EST libraries. Preliminary analysis of unreplicated gene expression data at three time points (0.5, 2, 6 hr) suggests that the early responses can be subdivided into four groups. Group I started highly upregulated at 0.5 hr but declined at 2 and 6 hrs. Most of the known genes in this group are those that are typically induced during pathogen invasion, wounding and other similar effects. Transcription factors, genes associated with protein chaperoning and degradation, signal transduction proteins and reactive oxygen scavengers are also represented. This pattern suggests the expression of rapid response genes that are either part of the initial phase of the defense pathway or general responses to temperature shock. Group 2 started slightly upregulated at 0.5 hr but declined at 2 and 6 hrs. Genes in this group appear to be involved with signal transduction, transcriptional activation of stress-related genes and cell membrane modification. Group 3 is slightly to highly upregulated at 0.5 hr followed by a continuous decline at 2 and 6 hrs. Genes in this group appear to be involved with signal transduction. Group 4 is upregulated at 6 hr. Many of the known genes in this group appear to be involved with protein synthesis and cellular transport.