Technical Abstract: Legume flavonoids have received considerable attention due to their beneficial effects to human health. Various results have been reported on soybean, but little research work has been conducted on other legumes. In this report, flavonoids (including daidzein, genistein, kaempferol, myricetin, and quercetin) in different legume seeds (peanut, lablab, cowpea, mung bean and soybean with different seed-coat colors) were quantified by HPLC. The flavonoid content with each accession was compared by statistical analysis. On average, soybean seeds contained significantly higher amounts of daidzein (335 ug/g) genistein (448 ug/g) and kaempferol (52 ug/g) while cowpea seeds contained a significantly higher amount of myricetin (86 ug/g) than the other four legumes. Cowpea and peanut seeds contained significantly higher amounts of quercetin (247 ug/g and 211 ug/g) than the other three legumes. Within the same species, the difference in quantity of a specific flavonoid among accessions varied greatly (from 2-285 fold). Association of seed-coat color with flavonoid content in the seeds across species was difficult due to complexity of seed-coat color classification, except that seeds with a black seed-coat contained a higher amount of myricetin. Daidzein was significantly correlated with genistein and kaempferol (r=0.93248, P<0.0001;r =0.58246, P<0.0001), respectively. Genistein was also significantly correlated with kaempferol (r=0.72547, P<0.0001). The variatioin in the flavonoid content detected and seed-coat color observed in this study would be useful for legume breeders and consumers.