|Jadhao, Samadhan - USDA-ARS-SEPRL|
Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: January 18, 2006
Publication Date: July 16, 2006
Citation: Jadhao, S.J., Lee, C.W., Suarez, D.L. 2006. Protective efficacy of inactivated influenza vaccines against highly pathogenic Asian H5N1 avian influenza viruses in chickens [abstract]. American Association of Avian Pathologists. CD p. 77. Technical Abstract: We evaluated the efficacy of vaccines prepared from Asian and American avian influenza viruses (AIVs) to protect chickens from lethal challenge with highly pathogenic (HP) Asian H5N1 AIVs. Vaccination studies were conducted in two week old chickens using inactivated vaccines prepared from reverse genetics rescued viruses or American wild type viruses, A/Turkey/Wisconsin/68 (H5N9) or A/Chicken/Mexico/232/94 (H5N2). The reverse genetics viruses used the hemagglutinin gene of A/Chicken/Indonesia/7/2003, a HP virus that was modified to be low pathogenic with different neuraminidase and internal genes. Oro-nasal challenge of vaccinated birds with a lethal dose of either A/Chicken/Indonesia/07/03 or A/Chicken/Supuranburi/2/04 virus showed all three vaccines provided good protection for chickens. Additionally, some of the vaccines could be used to differentiate infected from vaccinated animals because of different neuraminidase antibody responses.