Submitted to: Current Protocols in Microbiology
Publication Type: Book / Chapter
Publication Acceptance Date: January 27, 2006
Publication Date: N/A
Technical Abstract: A laboratory protocol was developed for inclusion in a routinely updated comprehensive manual of state-of-the-art research methods used in the study of microorganisms. Protocols provide step-by-step instructions and clear methodologies to allow researchers and students to conduct experiments. This protocol describes how to use aphids, or other sap-sucking insects, to transmit viruses either from infected plant material or by using an in vitro membrane system that allows insects to feed on purified virus alone or in combination with other purified proteins. Transmission protocols are provided for the short duration transmission of nonpersistent viruses and the longer duration transmission of semipersistent or persistent viruses. Additionally, general information is provided on rearing aphids for use in virus transmission experiments.