Technical Abstract: Campylobacter spp. are known to cause acute bacterial gastroenteritis in humans. Poultry products have been implicated as a significant source of these infections. Six experiments were performed to determine if Campylobacter spp. could be isolated naturally from the lymphoid-like tissues of commercial broiler breeder hens. Broiler breeder hens were acquired from different commercial sources during the early, middle, and late lay cycles. The birds were euthanized, de-feathered and aseptically opened. To reduce the possibility of cross-contamination between samples, the thymus, spleen, and liver/gallbladder were aseptically removed prior to the ceca. Individual samples were placed in sterile bags, packed on ice and transported to the laboratory for evaluation. In this study Campylobacter spp. were found in 11/43 thymus, 8/43 spleen, 4/43 liver/gallbladder, and 30/43 ceca. Overall, 28/53 isolates were Campylobacter coli and 25/53 isolates were found to be Campylobacter jejuni.