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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Characterization of Wheat-Alien Species Amphiploids and Chromosome Addition Lines Resistant to Fusarium Head Blight

Authors
item Mcarthur, Rachel - PLNT SCI NDSU, FARGO ND
item Oliver, Rebekah - PLNT SCI NDSU, FARGO ND
item XU, STEVEN
item Stack, Robert - PLNT PATH NDSU FARGO ND
item WANG, RICHARD
item Cai, Xiwen - PLNT SCI NDSU FARGO ND

Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 14, 2005
Publication Date: December 11, 2005
Citation: Mcarthur, R.I., Oliver, R., Xu, S.S., Stack, R., Wang, R., Cai, X. 2005. Molecular characterization of wheat-alien species amphiploids and chromosome addition lines resistant to fusarium head blight. Proceedings of 2005 National Fusarium Head Blight Forum , December 11-13, 2005. Milwaukee, WI. pp. 60.

Technical Abstract: Four Triticum aestivum L. cv. ‘Fukuhokomuji (Fuku)’- Elymus rectisetus (2n = 6x = 42, genomes StStYYWW) chromosome addition lines (2n = 44), seven T. aestivum L. cv. ‘Chinese Spring (CS)’- Thinopyrum junceum (2n = 6X = 42, genomes EbEbEbEbEeEe) amphiploids, and thirteen CS-Th. junceum addition lines (2n = 44) were evaluated for Type II resistance to Fusarium head blight (FHB). One Fuku-E. rectisetus addition line, A1034, three amphiploids,AJAP3, AJAP4, AJAP7, and two CS-Th. junceum addition lines, AJDAj2 and AJDAj3 exhibited resistance comparable to the resistant control ‘Sumai 3’. The mean FHB severity of these resistant lines was significantly lower than the susceptible controls CS and Russ, a common spring wheat cultivar. RFLP analysis indicated that the E. rectisetus chromosome in three of the four Fuku-E. rectisetus addition lines, A1026, A1048, and A1057, belonged to homoeologous group 1. In addition, Th. junceum chromosomes belonging to homoeologous groups 1, 2, and 4 were identified through RFLP analysis in the CS-Th. junceum addition lines, including AJDAj1, AJDAj2, AJDAj3, AJDAj4, AJDAj8, and HD3515. Characterization of low molecular weight glutenin subunits in the seeds by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) confirmed the RFLP results for the addition lines carrying a group 1 chromosome of E. rectisetus or Th. junceum. Chromosomes in the resistant amphiploids and addition are being further characterized using fluorescence in situ hybridization (FISH). These resistant lines could serve as a novel source of FHB resistance for wheat breeding. Understanding of their genetic constitutions will enhance the utilization of these resistance sources in the development of wheat varieties resistant to this devastating disease.

Last Modified: 8/19/2014
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