ARS | Publication request: EFFECTS OF LEPTIN ON CONCANAVALIN A (CONA) STIMULATED LYMPHOCYTE FUNCTION IN VITRO

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: November 4, 2005
Publication Date: November 5, 2005
Citation: Williams, Paige N., Laurenz, J.C., Carroll, J.A., Welsh, T.H. 2005. Effects of Leptin on Concanavalin A (ConA) stimulated Lymphocyte function in vitro. Available: http://www.tamus.edu/pathways/documents/Symposium/abstracts/2005/agriculture.doc.

Technical Abstract: Effects of Leptin on Concanavalin A (ConA) stimulated Lymphocyte function in vitro. Williams*1, P.N., J.A. Carroll2, T.H. Welsh, Jr.3 and J..C. Laurenz11Texas A&M University-Kingsville, 2USDA-ARS Livestock Issues Research Unit, Lubbock, TX., 3Texas A&M University-College Station This study investigated the time-and-dose dependent effects of Leptin on ConA induced lymphoproliferation and IgM production in vitro. Blood was obtained from crossbred pigs via jugular venipuncture and lymphocytes isolated by density gradient centrifugation. As expected, ConA (0 to 10 µg/mL) induced time- and dose-dependent increases (P < 0.01) in lymphocyte proliferation and IgM production. Leptin did not affect (P > 0.05) lymphocyte proliferation at any of the doses employed in this study (0, 1.7, 17, 170 ng/mL). At both 48 and 96 hours of culture Leptin had a modest suppressive effect (P < 0.05) on IgM production. These results indicate that Leptin can modulate specific aspects of lymphocyte function, but not all.