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United States Department of Agriculture

Agricultural Research Service

Title: Evaluation of Three Different Methods for Antimicrobial Susceptibility Screenin of Campylobacter

Authors
item Wallace, Frederick
item Cray, Paula

Submitted to: American Society for Microbiology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 21, 2000
Publication Date: May 21, 2000
Citation: Wallace, F.M., Cray, P.J. 2000. Evaluation of three different methods for antimicrobial susceptibility screenin of campylobacter. American Society for Microbiology Meeting. Session 193/Z. Abstract Z-9. P. 685-686.

Technical Abstract: Antimicrobial resistance in foodborne pathogens, particularly Campylobacter and Salmonella, has emerged as a global problem. Reliable testing methods are required to accurately assess the development of resistance. The fastidious growth requirements and fragility of Campylobacter species present significant obstacles to the design of antimicrobial susceptibility screening methods relative to other enteric pathogens. We evaluated the susceptibility of 55 poultry carcass wash isolates of Campylobacter to 8 antibiotics (Azithromycin, Ciproflaxacin, Clindamycin, Chloramphenicol, Erythromycin, Gentamicin, Nalidixic Acid, and Tetracycline) by 3 different methods: agar dilution, microbroth dilution (Trek Diagnostics), and the E-test (AB Biodisk). Agar dilution assays were conducted using Mueller Hinton Agar with blood, and microbroth dilution assays were conducted according to manufacturers protocols using Mueller Hinton Broth or HTM Broth. Overall, both the microbroth dilution and the E-test produced results highly comparable to the agar dilution technique. The agar dilution technique, however, was the most labor intensive while the microbroth dilution technique was the least labor intensive. Optimization of the microbroth dilution method was effected which increased sample throughput and reproducibility. The efficiency of our microbroth dilution assay shows significant promise for routine susceptibility testing of Campylobacter.

Last Modified: 9/20/2014