Technical Abstract: The objective of this paper is to describe the program PyPedal (v 2.0) and provide computational details for some of its key metrics. The package provides tools for pedigree analysis, report generation, and data visualization. Metrics include coefficients of inbreeding and relationship, effective founder and ancestor numbers, and founder genome equivalents. Tools are provided for identifying ancestors and descendants, computing coefficients of inbreeding from potential matings, quantifying pedigree completeness, and visualizing pedigrees. Scripting support is provided by the Python programming language and may be used to easily automate analyses and implement new features. Input and output files utilize plain-text formats. PyPedal runs on the GNU/Linux and Microsoft Windows operating systems. The program, documentation, and examples of usage are available on the World Wide Web at http://pypedal.sourceforge.net/. PyPedal has been applied to the pedigrees for a colony of dog guides and the U.S. Ayrshire population.