Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 10, 2005
Publication Date: January 8, 2008
Citation: Liu, J., Stipanovic, R.D., Bell, A.A., Puckhaber, L.S. 2008. Gossypol pathway in cotton: Purification and characterization of (+)-gossypol-forming dirigent protein from moco cotton [abstract]. Proceedings of Beltwide Cotton Conferences, January 8-12, 2008, Nashville, Tennessee. 2008 CDROM. Technical Abstract: Gossypol is biosynthesized by the free radical coupling of two molecules of hemigossypol to yield two optically active enantiomers, (+)-gossypol and (-)-gossypol. We have demonstrated that the preferential (+)-gossypol formation in moco cotton is mediated by a (+)-gossypol-forming dirigent protein which controls the stereospecificity of peroxidative coupling of two molecules of hemigossypol by a peroxidase. Stereospecific coupling of high energy free radicals is a poorly understood process. The purification and characterization of the (+)-gossypol-forming dirigent protein will further elucidate this important process. Purification of the moco cotton(+)-gossypol-forming dirigent protein is accomplished by anion exchange, gel filtration and affinity column chromatography. The properties of the purified dirigent protein are also characterized.