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United States Department of Agriculture

Agricultural Research Service

Title: The Endo-Beta-Mannanase Gene Families in Arabidopsis, Rice and Poplar

Authors
item Yuan, Joshua - UNIV OF TENN-KNOXVILLE
item Koizuka, Nobuya - OREGON ST UNIV-CORVALLIS
item Yang, Xiaohan - UNIV OF TENN-KNOXVILLE
item Lai, Inru - UNIV OF TENN-KNOXVILLE
item Lin, Hong
item Cheng, Zong-Ming - UNIV OF TENN-KNOXVILLE
item Nonogaki, Hiroyuki - OREGON ST UNIV-CORVALLIS
item Chen, Feng - UNIV OF TENN-KNOXVILLE

Submitted to: Functional and Integrative Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 19, 2006
Publication Date: August 8, 2006
Citation: Yuan, J.S., Koizuka, N., Yang, X., Lai, I., Lin, H., Cheng, Z., Nonogaki, H., Chen, F. 2006. The Endo-beta-mannanase Gene Families in Arabidopsis, Rice and Poplar. Functional and Integrative Genomics. 7:1-16.

Interpretive Summary: Mannan polysaccharides are important components of plant cell walls, and are especially rich in endosperm cell walls of many seeds. Degradation of mannans involves several types of enzymes, one of which is endo-beta-mannanase (EC 3.2.1.78), which randomly hydrolyzes the internal beta-1,4-D-mannopyranosyl linkage in the backbone of mannan polymers. In plants, endo-beta-mannanase has been relatively well studied for its function in seed germination and post-germinative reserve mobilization. Its involvement in other plant biological processes, however, remains poorly characterized. With the completed genome sequences of Arabidopsis thaliana, poplar and rice, we report the in silico identification of endo-beta-mannanase gene families in these three species. Divergent expression patterns of individual genes from this study suggest that the enzymes encoded by these genes are involved in diverse biological processes.

Technical Abstract: Mannan polysaccharides are important hemicellulosic components of plant cell walls, and are especially rich in the endosperm cell walls of many seeds. Degradation of mannans involves several types of enzymes, one of which is endo-beta-mannanase (EC 3.2.1.78), which randomly hydrolyzes the internal beta-1,4-D-mannopyranosyl linkage in the backbone of mannan polymers. In plants, endo-beta-mannanase has been relatively well studied for its function in seed germination and post-germinative reserve mobilization. Its involvement in other plant biological processes, however, remains poorly characterized. With the completed genome sequences of Arabidopsis thaliana, poplar and rice, in this paper, we report the in silico identification of endo-beta-mannanase gene families in these three species, which contains eight, eleven, and nine endo-beta-mannanase genes respectively. Genomic organization and phylogenic analysis of the A. thaliana and rice endo-beta-mannanase genes suggest that segmental duplication has played a more important role than tandem duplication in the evolution of endo-beta-mannanase gene family in both species. Further phylogenetic analysis including the endo-beta-mannanase sequences from bacteria, fungi, animals and other plant species implies that endo-beta-mannanase genes have an ancient evolutionary origin. Expression analysis of the A. thaliana and rice endo-beta-mannanase genes using RT-PCR was performed. Divergent expression patterns of individual genes suggest that the enzymes encoded by these genes are involved in diverse biological processes.

Last Modified: 7/24/2014
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