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United States Department of Agriculture

Agricultural Research Service

Title: Cryopreservation of Arabidopsis Thaliana Shoot Tips.

Authors
item Towill, Leigh
item Volk, Gayle

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2005
Publication Date: July 1, 2005
Citation: Towill, L.E., Volk, G.M. 2005. Cryopreservation of arabidopsis thaliana shoot tips [abstract]. Hortscience. 40(4):1067

Interpretive Summary: Arabidopsis thaliana shoot tips provide a model to study processes important for cryopreservation. Cryopreservation was accomplished using both vitrification and two-step cooling methods. With vitrification methods, shoot formation after liquid nitrogen exposure was 100% and 95%, respectively, for shoot tips exposed to PVS2 at 0°C and to PVS3 at 23°C. Cold acclimation of stock plants did not affect the percent of shoot tips growing after LN exposure. A two-step cooling method also gave greater than 90% survival if shoot tips were first cooled at 0.3°C min-1 below –30°C before immersing the samples into liquid nitrogen.

Technical Abstract: Arabidopsis thaliana shoot tips provide a model to study processes important for cryopreservation. Cryopreservation was accomplished using both vitrification and two-step cooling methods. With vitrification methods, shoot formation after liquid nitrogen exposure was 100% and 95%, respectively, for shoot tips exposed to PVS2 at 0°C and to PVS3 at 23°C. Cold acclimation of stock plants did not affect the percent of shoot tips growing after LN exposure. A two-step cooling method also gave greater than 90% survival if shoot tips were first cooled at 0.3°C min-1 below –30°C before immersing the samples into liquid nitrogen.

Last Modified: 11/25/2014
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