ARS | Publication request: MULTI-LOCUS SSR MARKERS FOR GENOTYPING AND ASSESSING GENETIC DIVERSITY OF XYLELLA FASTIDIOSA IN CALIFORNIA

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: October 1, 2005
Publication Date: December 5, 2005
Citation: Lin, H., Civerolo, E.L., Groves, R.L., Walker, A. 2005. Multi-locus Simple Sequence Repeat (SSR) Markers for Genotyping and Assessing Genetic Diversity of Xylella fastidiosa in California. Proceedings for the 2005 Pierce's Disease Research Symposium. p. 170-172.

Interpretive Summary: DNA identification system for Xyllela fastidiosa (Xf) was designed and developed. 34 of DNA typing markers were validated and are available to the public. These primers are Xf-specific and powerful for detecting genetic variations among and within crop-associated Xf strains and can be used for Xf identification, population structure and genetic diversity studies. Fluorescent-labeled primers were used in PCR with a rapid sample preparation protocol and with an automatic ABI 3100 genetic analyzer to create a high-throughput Xf pathogen diagnostic and genetic analysis platform. This marker system was used to study the geographic population structures of grape Xf strains in California, and as a tool to study interactions between Vitis and Xf in PD resistant and susceptible grapes.

Technical Abstract: We have designed and developed Xyllela fastidiosa (Xf) Simple Sequence Repeat (SSR) primers. 34 of them have been validated and are available to public (Lin et al. 2005). These primers are Xf-specific and powerful for detecting polymorphism among and within crop-associated Xf strains and can be used for Xf genotyping, population structure and genetic diversity studies. Recently, we used fluorescent-labeled primers for PCR and an ABI 3100 genetic analyzer in combination with our rapid sample preparation protocol to create a high-throughput Xf pathogen diagnostic and genetic analysis platform. We used this marker system to study the geographic population structures of grape Xf strains in California. We also used this marker system as a tool to study interactions between Vitis and Xf in PD resistant and susceptible grapes.