Location: Cool and Cold Water Aquaculture Research
Title: Characterization of Major Histocompatibility (Mh) Haplotypes and Their Association with Disease Resistance in Rainbow Trout Authors
Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 2005
Publication Date: December 15, 2005
Citation: Palti, Y., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J. 2005. Characterization of major histocompatibility (MH) haplotypes and their association with desease resistance in rainbow trout. Plant and Animal Genome Conference. Abstract ID P643, page 262. Technical Abstract: Four unlinked major histocompatibility (MH) regions of the rainbow trout genome were previously mapped and sequenced. For each locus we have identified several microsatellites and developed markers to enable characterization of MH haplotypes in our broodstock and to identify association of those haplotypes with resistance to Yersinia ruckeri, a gram negative bacterium that causes Enteric Redmouth Disease (ERM), an acute hemorrhagic septicemia. Ninety eight full-sib families were challenged by immersion exposure in one to four replicates (50 fish per replicate), and the mortality rate was monitored for 19 days. The 15 families with highest survival rates and 14 with lowest survival rates were genotyped using 12 MH microsatellites. Significant association (P<0.05) was detected between the MH class II marker OMM3027 and disease resistance by comparing allele frequencies between the high and low parents. Associations of MH II markers to disease resistance were also detected within informative families with segregating alleles. Significant linkage disequilibrium (LD) was detected within the MH II markers and within markers derived from the TAP1 region, and between MH II and TAP1 markers. Notable (P<0.10) LD was detected between MH II and MH IB markers and between TAP1 and MH IB markers.