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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFICATION AND CHARACTERIZATION OF GENES AFFECTING COOL AND COLD WATER AQUACULTURE PRODUCTION

Location: Cool and Cold Water Aquaculture Research

Title: Effects of Recombinant Bovine Somatotropin (Rbst) on the Liver and Muscle Transcriptomes of High Growth Rate Rainbow Trout (Oncorhynchus Mykiss)

Authors
item Gahr, Scott
item WEBER, GREGORY
item SHEPHERD, BRIAN
item SILVERSTEIN, JEFF
item VALLEJO, ROGER
item Coulibaly, Issa - WV UNIVERSITY
item REXROAD, CAIRD

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 2005
Publication Date: December 15, 2005
Citation: Gahr, S.A., Weber, G.M., Shepherd, B.S., Silverstein, J., Vallejo, R.L., Coulibaly, I., Rexroad III, C.E. 2005. Effects of Recombinant Bovine Somatotropin (rbst) on the Liver and Muscle Transcriptomes of High Growth Rate Rainbow Trout (Oncorhynchus Mykiss). Plant and Animal Genome Conference, Jan 2006. Abstract ID P534, pg 260.

Technical Abstract: Numerous studies have established that recombinant bovine Somatotropin (rbST) will stimulate growth in the rainbow trout. However, the effects of rbST on target tissue gene expression are not well characterized. In the current study, we used Posilac® (Monsanto Co., St. Louis, MO) to assess the effects of an rbST implant on liver and muscle transcript levels in fish selected for high growth rate. Twelve fish (~100 g bw) from each of two families selected for their high growth rates were placed into individual tanks. Following a two week acclimation period, four fish from each family received an injection of Posilac® (120 micro g/g BW) and four received an equivalent volume of the carrier (~50 micro l). Three days after the injection, fish were euthanized and tissue and blood samples were collected. Blood IGF-I levels were used to verify increased growth hormone activity in the rbST treated fish. Alterations of muscle and liver transcriptomes by rbST were characterized using the MSU 1RT rainbow trout and GRASP 16K Atlantic salmon cDNA microarrays. Carrier injected samples were randomly paired with rbST injected samples within each family using a dye swap microarray design. Differentially-expressed transcripts will be explored for their utility as candidate genes associated with growth and development in the rainbow trout.

Last Modified: 9/10/2014