ARS | Publication request: ANALYSIS OF THE SWINE TRANSCRIPTIONAL RESPONSE TO SALMONELLA ENTERICA SEROVAR TYPHIMURIUM

Submitted to: American Society for Microbiology Branch Meeting
Publication Type: Abstract Only
Publication Acceptance Date: September 22, 2005
Publication Date: September 22, 2005
Citation: Uthe, J.J., Royaee, A., Lunney, J.K., Stabel, T.J., Zhao, S., Tuggle, C.K., Bearson, S.M. 2005. Analysis of the swine transcriptional response to Salmonella enterica serovar Typhimurium [abstract]. North Central Branch-American Society for Microbiology. p. 69.

Technical Abstract: S. Typhimurium is a foodborne pathogen with the potential to infect a wide range of hosts including birds, reptiles and mammals. In swine, S. Typhimurium usually causes enterocolitis of variable severity with clinical symptoms of the disease lasting for about a week. However, some swine can become chronically infected carriers of the organism and, under conditions of stress, can shed S. Typhimurium, causing a serious contamination issue on the farm and at the slaughter plant. In order to gain a better understanding of the interactions of S. Typhimurium with its swine host, transcriptional profiling of the porcine response to S. Typhimurium infection was performed. Real-time PCR of swine genes involved in Th1- and Th2-mediated immunity, innate/inflammatory responses, T-cell and cytoskeleton regulation, heat shock response and chaperone functions was performed on RNA from the mesenteric lymph nodes of the S. Typhimurium-infected swine over the time course of infection (8 hours to 21 days). Analysis revealed that swine inoculated with S. Typhimurium initiated only a transient up-regulation of the immune markers involved in the Th1-mediated response (IFNG, INDO, STAT1, IL12, SOCS1, IRF1, CD47/IAP, CXCL10) as well as genes encoding for cytoskeleton regulation (ARPC2), heat shock proteins and chaperones (HSPH1 and VCP) and transmembrane glycoprotein (SCARB2). Th2-mediated and innate/inflammatory responses (IL4, IL6, TRL4, CSF2) were generally repressed in the S. Typhimurium-infected swine. Therefore, S. Typhimurium induces a weak induction of transcriptional response in the pig that may be sufficient to fight the infection but not eliminate the pathogen, potentially aiding S. Typhimurium to progress into a carrier state in the pig and serve as a contamination risk and food safety concern.