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ARS Home » Plains Area » Sidney, Montana » Northern Plains Agricultural Research Laboratory » Pest Management Research » Research » Publications at this Location » Publication #185386

Title: POLYMORPHIC MICROSTAELLITE MARKERS IN POLYPLOID LEPIDIUM DRABA L. SSP. DRABA (BRASSICACEAE) AND CROSS-SPECIES AMPLIFICATION IN CLOSELY RELATED TAXA

Author
item BON, MARIE-CLAUDE - EBCL-ARS
item HURARD, C. - EBCL-ARS
item Gaskin, John
item RISTERUCCI, A.M. - CIRAD

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/29/2004
Publication Date: 3/1/2005
Citation: Bon, M., Hurard, C., Gaskin, J.F., Risterucci, A. 2005. Polymorphic microstaellite markers in polyploid Lepidium draba L. ssp. draba (Brassicaceae) and cross-species amplification in closely related taxa. Molecular Ecology Notes. 5(1):68-70.

Interpretive Summary: At present, efficient biological control of this Eurasian native weed in the USA is hampered by lack of knowledge of its population genetic structure and colonization process. Here, we describe the development of 11 polymorphic microsatellite markers that also reveal the polyploidy of this weed. Successful cross-species amplification highlights the possibility of using these markers for genetic studies in other closely related species.

Technical Abstract: Heart-podded hoary cress, Lepidium draba L. ssp. draba (Brassicaceae) is a noxious invasive weed in the USA. DNA markers were developed to help discriminate various types of hoarycress. With these markers, Eurasian origins of this invasive weed can be determined, and this will help us find potential biological control agents that have evolved with these plants.