Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: August 1, 2005
Publication Date: October 1, 2005
Citation: Mott, I.W., Wang, R., Chatterton, N.J. 2005. Transcriptome Analyses of Salt-Tolerant W4090 and W4910 Wheat Germplasm Lines [Abstract}.. ASA-CSSA-SSSA Annual Meeting Abstracts. Technical Abstract: Salt-tolerant wheat lines W4909 amd W4910 were derived from a cross between AJDAj5 (a disomic addition line carrying a pair of Eb chromosomes from Thinopyrum junceum) and Ph1 (a line having the Ph1 allele from Aegilops speltoides). Both lines have greater salt-tolerance than their parental lines, which are more salt-tolerant than the common wheat background, Chinese Spring. Genomic constitution of W4909 and W4910 wheat has been estimated at 1.9% and 2.4% non-Chinese Spring, respectively. cDNA subtractive suppression hybridization (SSH) was used to identify differentially expressed genes under salt stress at EC=36 dS/m. Of 768 screened root cDNAs, 138 clones were found to represent two genes, 4910D4 (132 clones) and 4909D4 (six clones). Of 384 leaf cDNAs that were screened, 41 clones were found to represent two genes, 4910D4 (27 clones), the same gene that expressed in roots, and 4910M8 (14 clones). Northern analysis and RT-PCR confirmed that 4910D4 (an unknown gene transcript) was absent in Chinese Spring roots but expressed in W4909 and W4910 at a two-fold level greater than in their parental lines. Differential expression of 4909D4 and 4910M8 (similar to genes coding for putative receptor-line protein kinase and sulfur-rich/thionin-like protein, respectively) was confirmed with RT-PCR. Additionally, PCR primer pairs designed from 4910D4 and 4909D4 sequences were shown to amplify a target fragment from genomic DNA of W4909, W4910, Ph1 and AJDAj5, but not Chinese Spring.