FATE AND TRANSPORT OF MANURE-BORNE PATHOGENIC MICROORGANISMS
Title: THE EFFECTS OF E-BEAM IRRADIATION ON EASTERN OYSTERS (CRASSOSTREA VIRGINICA) EXPERIMENTALLY INFECTED WITH CRYPTOSPORIDIUM PARVUM
| Collins, Marina - VA TECH,BLACKSBURG,VA |
| Flick, George - VA TECH,BLACKSBURG,VA |
| Lindsay, David - VA TECH,BLACKSBURG,VA |
| Smith, Stephen - VA-MD VET MED, VA TECH |
| Jahncke, Michael - VA TECH,BLACKSBURG,VA |
| Croonenberghs, Robert - VA DEPT. OF HEALTH |
| O'Keefe, Sean - VA TECH,BLACKSBURG,VA |
Submitted to: Institute of Food Technology
Publication Type: Abstract Only
Publication Acceptance Date: August 18, 2005
Publication Date: September 15, 2005
Citation: Collins, M., Flick, G., Lindsay, D., Fayer, R. 2005. The Effects of E-Beam Irradiation on Eastern Oysters (Crassostrea virginica) Experimentally Infected with Cryptosporidium parvum [abstract]. Institute of Food Technologists Nonthermal Processing Division Meeting, September 2005, Philadelphia, PA.
Shellfish have been identified as a potential source of Cryptosporidium infection for humans. The inactivation of C. parvum and other pathogens in raw molluscan shellfish would provide increased food safety for normal and at-risk consumers. The present study identified the efficacy an alternative food-processing treatment, e-beam irradiation, on the viability of C. parvum oocyts in Eastern oysters (Crassostrea virginica). Oysters were artificially infected with the Beltsville strain of C. parvum and subjected to e-beam and microwave treatments. The effects of the treatments were evaluated by inoculation of the processed oyster tissues using the neonatal mice assay. Significant reductions (P < 0.05) in infectivity were observed for in-shell and shucked oysters treated with e-beam irradiation doses of 1.0, 1.5 or 2 kGy. A dose of 2 kGy completely terminated C. parvum infectivity and did not adversely affect the visual appearance of the oysters. The FDA approved a petition on the application of irradiation for pathogen control in shellfish during the week of August 14, 2005.