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United States Department of Agriculture

Agricultural Research Service

Research Project: FATE AND TRANSPORT OF MANURE-BORNE PATHOGENIC MICROORGANISMS Title: The Effects of E-Beam Irradiation on Eastern Oysters (Crassostrea Virginica) Experimentally Infected with Cryptosporidium Parvum

Authors
item Collins, Marina - VA TECH,BLACKSBURG,VA
item Flick, George - VA TECH,BLACKSBURG,VA
item Lindsay, David - VA TECH,BLACKSBURG,VA
item Fayer, Ronald
item Smith, Stephen - VA-MD VET MED, VA TECH
item Jahncke, Michael - VA TECH,BLACKSBURG,VA
item Croonenberghs, Robert - VA DEPT. OF HEALTH
item O'Keefe, Sean - VA TECH,BLACKSBURG,VA

Submitted to: Institute of Food Technology
Publication Type: Abstract Only
Publication Acceptance Date: August 18, 2005
Publication Date: September 15, 2005
Citation: Collins, M., Flick, G., Lindsay, D., Fayer, R. 2005. The Effects of E-Beam Irradiation on Eastern Oysters (Crassostrea virginica) Experimentally Infected with Cryptosporidium parvum [abstract]. Institute of Food Technologists Nonthermal Processing Division Meeting, September 2005, Philadelphia, PA.

Technical Abstract: Shellfish have been identified as a potential source of Cryptosporidium infection for humans. The inactivation of C. parvum and other pathogens in raw molluscan shellfish would provide increased food safety for normal and at-risk consumers. The present study identified the efficacy an alternative food-processing treatment, e-beam irradiation, on the viability of C. parvum oocyts in Eastern oysters (Crassostrea virginica). Oysters were artificially infected with the Beltsville strain of C. parvum and subjected to e-beam and microwave treatments. The effects of the treatments were evaluated by inoculation of the processed oyster tissues using the neonatal mice assay. Significant reductions (P < 0.05) in infectivity were observed for in-shell and shucked oysters treated with e-beam irradiation doses of 1.0, 1.5 or 2 kGy. A dose of 2 kGy completely terminated C. parvum infectivity and did not adversely affect the visual appearance of the oysters. The FDA approved a petition on the application of irradiation for pathogen control in shellfish during the week of August 14, 2005.

Last Modified: 4/23/2014
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