|Palencia, E - PLANT.PATH./U.GEORGIA|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2005
Publication Date: July 30, 2005
Citation: Palencia, E.R. and Glenn, A.E. 2005. FvABC1, a Fusarium verticillioides gene encoding an ABC transporter, may be required for tolerance of phytoanticipins produced by corn [abstract]. Phytopathology. 95(6):S79. Interpretive Summary: Abstract - no summary.
Technical Abstract: The phytopathogenic ascomycete Fusarium verticillioides is commonly found in corn (Zea mays) and is associated with the production of mycotoxins called fumonisins. The plant produces the phytoanticipins 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), yet F. verticillioides can metabolically detoxify these compounds. Although this detoxification may be important for fungal ecological fitness, it is not a major virulence factor. The present study was initiated to characterize an ATP-binding-cassette (ABC) transporter in F. verticillioides (FvABC1). ABC transporters are a large family of proteins involved in efflux of compounds from the fungal cell. Sequencing of FvABC1 showed the general structure of an ABC transporter in eukaryotes with 2 halves; each half contained a nucleotide binding domain (NBD) and six transmembrane segments (TMS). To further characterize this gene, a gene disruption construct was created and used to transform F. verticillioides wild type. Transformants were screened on PDA+BOA (1mg/ml) to identify those with no growth or delayed growth. Confirmation of gene disruption is underway using PCR and Southern hybridization.