ARS | Publication request: VESICLE-SURFACE INTERACTIONS ON SELF-ASSEMBLED MONOLAYER LEAD TO FLATTENED VESICLES: A QCM-D STUDY

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 21, 2005
Publication Date: October 21, 2005
Citation: Evans, K.O. 2005. Vesicle-surface interactions on self-assembled monolayer lead to flattened vesicles: a qcm-d study [abstract]. Proceedings of the 2005 Q-Sense Users Invitational Symposium. p. 6.

Technical Abstract: Recent research using quartz crystal microbalance with dissipation has focused on the interaction of phospholipid vesicles with various surfaces such as silicon dioxide, titanium dioxide, gold, and mica. It was recently shown that phospholipid vesicles of a binary mix containing a Zwitterionic lipid (phosphatidylcholine) at 50-80 mole% and an anionic liquid (phosphatidylserine) present at 20-50 mole% did readily fuse to form a single bilayer in the presence of calcium ions on two of these surfaces, silicon dixiode and mica. Present research at the National Center for Agricultural Utilization Research was focused on characterizing the supported-membrane formation of similar lipid system (phosphatidylcholine-phosphatidylglycerol) on gold with self-assembled monolayers composed of 50-50 mix of 11-mercaptoundecanoic acid and 11-mercapto-1-decanol. Presented work will show that despite the presence of the anionic lipid, phospholipid vesicles did not fuse to form a single bilayer. Instead, vesicles flatten with some amount of entrapped fluid retained. Also, the importance of calcium ions will be discussed.