Submitted to: Patent Application
Publication Type: Patent Application
Publication Acceptance Date: October 3, 2002
Publication Date: February 8, 2005
Citation: Piazza, G.J., Foglia, T.A., Nunez, A. 2005. Epoxidation of carbon-carbon double bond with membrane bound peroxygenase. U.S.Patent 6,852,315. Technical Abstract: A method has been discovered for the epoxidation of a compound having at least one carbon-carbon double bond, the method involves reacting a compound having at least one carbon-carbon double bond, a solvent, an oxidant, and membrane bound peroxygenase. Also discovered is a method for preparing the membrane bound peroxygenase involving grinding seeds containing peroxygenase to produce ground seeds, homogenizing the ground seeds in a buffer to form a slurry, centrifuging the slurry to produce a first supernatant, centrifuging the first supernatant to produce a second supernatant, and filtering said second supernatant through a protein-binding membrane filter to produce membrane bound peroxygenase; optionally the second supernatant is filtered through a hydrophilic membrane filter prior to filtering the second supernatant through a protein-binding membrane filter.