ARS | Publication request: INDUCTION OF DIFFERENTIAL GENE EXPRESSION IN PERILLUS BIOCULATUS NYMPHS FED A SUBOPTIMAL ARTIFICIAL DIET

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: July 21, 2005
Publication Date: July 22, 2005
Citation: Yocum, G.D., Coudron, T.A., Brandt, S.L. 2005. Induction of differential gene expression in perillus bioculatus nymphs fed a suboptimal artificial diet. Genbank. Accession numbers DR730531 to DR730536.

Technical Abstract: Using suppression subtractive hybridization, fragments of two artificial diet up-regulated and two down-regulated transcripts were isolated from the predatory Pentatomid Perillus bioculatus. A BlastX search found matches for only the two diet-upregulated clones, to the tyrosine-3-monooxygenase gene and the gene for the a chitin binding protein Gasp. The probe generated from the tyrosine-3-monooxygenase clone hybridized to two transcripts 2.3 and 1.2 kb in size. The two transcripts were differentially regulated: the 2.3 kb transcript was upregulated in the 1st and late 3rd instar diet-fed nymphs, whereas the 1.2 kb transcript was upregulated in the 2nd and early 3rd instar diet-fed nymphs. The Gasp gene was upregulated in late 3rd instar nymphs. A positive correlation was found between levels of expression of the isolated genes and the number of generations the insects had been reared on the artificial diet. GenBank accession numbers DR730531 to DR730536 USDA-ARS Red River Valley Agricultural Research Center, Biosciences Research Laboratory, 1605 Albrecht Boulevard, Fargo, ND 58105 USA USDA-ARS Biological Control of Insects Research Laboratory, 1503 S. Providence, Research Park., Columbia, MO 65203-3535 USA