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United States Department of Agriculture

Agricultural Research Service

Research Project: IPM TECHNOLOGIES FOR SUBTROPICAL INSECT PESTS Title: Phylogenetic Relationships of Anastrepha Suspensa (Diptera: Tephritidae) Inferred from Mitochondrial Cytochrome Oxidase I DNA Sequence Data

Authors
item Boykin, Laura
item Shatters, Robert
item Hall, David
item Burns, Reed - DEPT OF PLANT INDUSTRY
item Franqui, Rosa - BOTANICAL GARDEN, SJ, PR

Submitted to: Bulletin of Entomological Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 10, 2006
Publication Date: August 7, 2006
Citation: Boykin, L.M., Shatters, Jr, R.G., Hall, D.G., Burns, R.E., Franqui, R.A. 2006. Analysis of host preference and geographical distribution of Anastrepha suspensa (Diptera: Tephritidae) using phylogenetic analyses of mitochondrial cytrochrome oxidase I DNA sequence data. Bulletin of Entomological Research. 96:1-13.

Interpretive Summary: Anastrepha suspensa is an economically important pest, which is restricted to the Greater Antilles and southern Florida (the known distribution of A. suspensa). A. suspensa infests a wide variety of hosts including citrus, a multi-million dollar industry in Florida. The recent increase in observance of citrus infested with A. suspensa in Florida has raised questions regarding host-specificity of certain populations and genetic diversity of the pest throughout its geographic distribution. Cytochrome Oxidase I (COI) DNA sequence data was used to characterize the genetic diversity of A. suspensa from Florida and Caribbean populations reared from different host plants. There are no unifying genetic differences looking at one region of DNA based on host-plant use (including citrus), geography (Florida, Jamaica, Cayman Islands, Puerto Rico or Dominican Republic), or population sampled. Furthermore, there was no significant difference in the COI data from samples collected in Florida during 1935 and 1965 as compared to samples collected in 2004.

Technical Abstract: Anastrepha suspensa is an economically important pest, restricted to the Greater Antilles and southern Florida. A. suspensa infests a wide variety of hosts and is of quarantine importance in citrus, a multi-million dollar industry in Florida. A recent increase in observance of citrus infested with A. suspensa in Florida has raised questions regarding host-specificity of certain populations and genetic diversity of the pest throughout its geographic distribution. Cytochrome Oxidase I (COI) DNA sequence data was used to characterize the genetic diversity of A. suspensa from Florida and Caribbean populations reared from different host plants. Phylogenetic analyses of the mitochondrial COI gene from 107 A. suspensa samples shows that A. suspensa is a monophyletic group and there is one synapomorphic character that unites all A. suspensa from one group of A. fracterculus, a paraphyletic species complex. The most likely tree of the COI locus does not support monophyletic groups based on host-plant use (including citrus), geography (Florida, Jamaica, Cayman Islands, Puerto Rico or Dominican Republic), or population sampled. Furthermore, there was no significant difference in the COI data from samples collected in Florida during 1935 and 1965 as compared to samples collected in 2004.

Last Modified: 10/25/2014
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