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United States Department of Agriculture

Agricultural Research Service

Title: Monooxygenase System of Bacillus Megaterium Ala2 and Effect of Environmental Factors on the Production of Oxygenated Fatty Acides from Linoleic Acid

item Hou, Ching

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: October 23, 2005
Publication Date: October 29, 2005
Citation: Hou, C.T. 2005. Monooxygenase system of Bacillus megaterium ALA2 and effect of environmental factors on the production of oxygenated fatty acids from linoleic acid. Proceedings of the United States-Japan UJNR Cooperative Program in Natural Resources Food and Agriculture Panel. 34:159-165.

Technical Abstract: We identified [1] many novel oxygenated fatty acids produced from linoleic acid by strain ALA2. Recently, we reclassified strain ALA2 as Bacillus megaterium and opened a possible link with the well-studied catalytically self-sufficient P450 monooxygenase of Bacillus megaterium ATCC14581 (NRRL B-3712) and Bacillus subtilis strain 168 (NRRL B-4219). Preliminary results indicated that strain ALA2 possesses a similar oxygenase system oxidizing palmitic acid to omega-1, omega-2, and omega-3 hydroxy palmitic acids. To develop an industrial process for these new oxygenated fatty acids by strain ALA2, the effect of environmental factors on the production and their impact on the amount of various products were studied. Dextrose at 5 g/l was the optimum amount for the carbon source. A combination of 15 g yeast extract and 10 g tryptone showed good results as nitrogen sources. Among the metal ions tested, magnesium 2.0 mM, iron 0.5 mM, zinc 0.1 mM, nickel 0.01 mM, and cobalt 0.05 mM were the optimum concentrations for the reaction. Copper ions did not affect the production of oxygenated products; however, manganese ions inhibited the reaction. Addition of these metal ions did not alter the distribution of products. The optimum temperature and pH for the production of THOAs were 30° C and pH 6.5. Time course studies showed 40 - 48 hours is the optimum for the production of both THOAs. These data provide the basis for engineering scale-up production of these new products.

Last Modified: 4/22/2015
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