Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 24, 2005
Publication Date: December 1, 2005
Citation: Nonneman, D.J., Smith, T.P., Freking, B.A., Rohrer, G.A. 2005. Assignment of 12 genes to porcine chromosome 1q by linkage and radiation hybrid mapping. Anim. Genet. 36:532-533. 2005. Interpretive Summary: Several regions on pig chromosome 1 have been identified that affect growth, composition, carcass and reproductive traits related to the production of pork. In order to identify the genes affecting these traits, efforts have been made to develop comparative maps between human and pigs. Porcine chromosome 1 is similiar to human chromosomes 6, 9, 14, 15 and 18. The purpose of this study was to assign genes to the genetic and physical maps in swine, specifically in QTL regions for carcass and reproductive traits. These are regions where gene order differs compared to human and an attempt was made to define the locations of these rearrangements. Twelve genes located on human chromosomes 6, 9 and 15 were mapped by linkage or physically by a radiation hybrid panel to pig chromosome 1. The addition of these markers to the comparative map will help identify positional candidate genes for economically important traits.
Technical Abstract: Twelve genes located in quantitative trait locus (QTL) intervals for growth, carcass and reproductive traits were comparatively mapped on pig chromosome 1. Seven genes with human homologs on chromosomes 6 and 9 (SMPDL3A, RPL24, CREB3, AMBP, PAPPA, PTGS1, and SLC27A4) were placed on the MARC linkage map using single nucleotide polymorphisms (SNPs) and eight genes with human homologs on chromosomes 6, 9 and 15 (RPL24, NTRK3, TMEM2, PTGS1, CIZ1, SLC27A4, GFI1B and AGPAT2) were physically mapped using the IMpRH radiation hybrid panel; three genes were assigned to both maps. The mapping results were consistent with previous comparative maps and further identified areas of chromosomal rearrangements between pig and human. Four of these genes (PTGS1, PAPPA, SLC29A4 and AGPAT2) are positional candidate genes for carcass and reproductive QTL.