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United States Department of Agriculture

Agricultural Research Service

Title: Whole Genome Sequencing in the Cereal Rust Fungi

Author
item Szabo, Les

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 15, 2005
Publication Date: September 20, 2006
Citation: Szabo, L.J. 2005. Whole genome sequencing in the cereal rust fungi. Proceedings of "Global Landscapes" in Cereal Rust Control Conference. p. 21.

Technical Abstract: Puccinia graminis, the causal agent of stem rust, historically has been one the most devastating fungal pathogens of small grain cereal crops. In order to elucidate the mechanisms of pathogenicity and biotrophic lifestyle of P. graminis, a genomic project has been initiated. P. graminis f.sp. tritici, isolate CRL 75-36-700-3 that was selected for sequencing, is one of the parents used to develop a partial genetic map (Zambino et al., 2000). This isolate has an avirulent phenotype for the following 25 stem rust resistance genes (Sr6, Sr7b, Sr8a, Sr8b, Sr9b, Sr9d, Sr10, Sr11, Sr13, Sr14, Sr22, Sr24, Sr25, Sr26, Sr27, Sr28, Sr29, Sr30, Sr31, Sr32, Sr33, Sr34, Sr35, Sr36, and Sr37) and a virluent phenotype for 21 stem rust resistance genes (Sr5, Sr9a, Sr9e, Sr9g, Sr15, Sr18, Sr20, Sr21, SrKt2, SrLC, SrMcN, SrPl, SrPt, SrTt-3, and SrWst-2). A shotgun sequencing strategy is being used with three random sheared libraries (4kb, 10kb, and 40kb), and to date 8X coverage has been sequenced. To help with assembly of the genome, approximatley 24,000 fosmid clones are in the process of being restriction printed. In addition, an EST project has been started. Four libraries have been constructed from urediniospores (urediniospores and germinated urediniospores; 8 and 24 hours) and teliospores. The goal of the EST project is to sequence at least 40,000 cDNA clones, each of which will be sequenced from both ends. Additional cDNA libraries will be made from isolated haustoria, aecia/pycnia spores, and infected leaf tissue (both wheat and barberry). Current progress of the Puccinia graminis genome sequencing project will be discussed as well as other rust fungal genome projects.

Last Modified: 9/23/2014