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United States Department of Agriculture

Agricultural Research Service

Title: Use of a Multiplex Rt-Pcr to Differentiate Lentogenic Ndv from End

item Sellers, Holly - UNIV OF GA - ATHENS, GA
item Linnemann, Erich - UNIV OF GA - ATHENS, GA
item Kapczynski, Darrell

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: February 8, 2005
Publication Date: July 16, 2005
Citation: Sellers, H.S., Linnemann, E.G., Kapczynski, D.R. 2005. Use of a multiplex RT-PCR to differentiate lentogenic NDV from END [abstract]. American Association of Avian Pathologists. p. 32.

Technical Abstract: A multiplex RT-PCR has been designed to differentiate lentogenic Newcastle disease viruses from exotic Newcastle disease (END). Using computer sequence analysis and published sequences (GenBank), we have constructed lentogenic (B1/LaSota) and END (CA02)-specific primer sets. The primers amplify regions within the phosphoprotein and fusion protein genes of NDV. The primer sets have been tested in uniplex RT-PCR assays and amplify the predicted target templates. Our preliminary evaluation of the primers in a multiplex RT-PCR format suggests the primers will be effective in discriminating lentogenic and velogenic NDVs. The multiplex RT-PCR parameters have been optimized. RNA from archived NDV field isolates and END experimentally infected and uninfected chickens will be tested. The sensitivity and specificity is currently being determined

Last Modified: 4/18/2015
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