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United States Department of Agriculture

Agricultural Research Service

Title: Colombian Datura Virus Diagnosed in Brugmansia Spp. in Florida

Authors
item Adkins, Scott
item Chellemi, Daniel
item Annamalai, M. - FDACS-DPI
item Baker, C. A. - FDACS-DPI

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2005
Publication Date: June 30, 2005
Citation: Adkins, S., Chellemi, D.O., Annamalai, M. Baker, C.A. 2005. Colombian datura virus diagnosed in Brugmansia spp. in Florida. Phytopathology. 95:S2.

Technical Abstract: Angel’s trumpet (Brugmansia spp.), also known as brugmansia, is a perennial shrub in the Solanaceae. Originally from South America, brugmansia is popular as a landscape plant in the tropics and subtropics and as a container plant in temperate regions. It is characterized by fragrant, trumpet-shaped flowers of various colors. Virus-like symptoms including a mosaic of light and dark green were observed on the leaves of several brugmansia plants in a south Florida nursery in November 2003. Inclusion body morphology and electron microscopy of leaf dips indicated the presence of a potyvirus. Supporting evidence was obtained by testing with a commercially-available enzyme-linked immunosorbent assay for multiple potyvirus species. Sequence analysis of ~1.7 kb 3’ terminal virus genome fragments amplified by reverse transcription-polymerase chain reaction from total RNA showed 99% nucleotide identity to the corresponding region of Colombian datura virus (CDV) sequences in GenBank. This confirmed the diagnosis and represents the first report of CDV infection of brugmansia (or any other species) in Florida. CDV was detected in Brugmansia spp. elsewhere in the United States 35 years ago but was considered no longer present. A broader survey is in progress.

Last Modified: 9/10/2014
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