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United States Department of Agriculture

Agricultural Research Service

Title: Applications of Genechips for Barley Improvement

Authors
item Muehlbauer, Gary - UNIVERSITY OF MINNESOTA
item Garvin, David
item Smith, Kevin - UNIVERSITY OF MINNESOTA
item Boddhu, Jayanand - UNIVERSITY OF MINNESOTA
item Seung, Cho - UNIVERSITY OF MINNESOTA

Submitted to: North American Barley Research Workshop Proceedings
Publication Type: Proceedings
Publication Acceptance Date: June 15, 2005
Publication Date: July 17, 2005
Citation: Muehlbauer, G.J., Garvin, D.F., Smith, K., Boddhu, J., Seung, C. 2005. Applications of genechips for barley improvement. North American Barley Research Workshop Proceedings, Red Deer, Alberta. p. 143-147.

Technical Abstract: The use of RNA profiling has become a powerful strategy to examine genome-wide gene transcript accumulation patterns. The Barley1 Affymetrix GeneChip probe array is a resource that can be used to examine the expression of greater than 20,000 genes in parallel. This article summarizes several research projects that are using the Barley1 Affymetrix GeneChip for a diverse range of studies. This includes physically mapping large numbers of barley genes to individual chromosomes and chromosome arms in wheat-barley aneuploid stocks, examining RNA profiles in barley infected with Fusarium graminearum, and identifying markers for specific barley genome regions in a targeted manner. The recent development of the Barley1 GeneChip probe array has provided the barley genetics community a new high-throughput tool to assess gene expression patterns. Thus, the Barley1 GeneChip probe array provides the barley genetics community a new high-throughput tool to assess gene expression patterns for a variety of purposes.

Last Modified: 10/22/2014
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