Title: Molecular Characterization of Delta-9 Desaturase 1 from the Glassy-Winged Sharpshooter, Homalodisca Coagulata (Hemiptera: Cicadellidae) Authors
|Cleland, C - LOS ALAMOS NAT RES LAB|
Submitted to: Florida Entomological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 22, 2005
Publication Date: July 24, 2005
Citation: Katsar, C.S., Hunter, W.B., Cleland, C.A. 2005. Molecular characterization of Delta-9 Desaturase 1 from the Glassy-winged Sharpshooter, Homalodisca Coagulata (Hemiptera: Cicadellidae). 88th annual meeting of the Florida Entomological Society. July 24-27, 2005, Ft. Myers, FL. Paper No. DSP-12. Technical Abstract: We report the isolation and characterization of a delta-9 desaturase from two Hemipterans, the Glassy-winged Sharpshooter, Homalodisca coagulata, and Oncometopia nigricans, two leafhoppers known to transmit Pierce’s Disease of grapes. The cDNA isolated encodes for a 367 amino acid belonging to Family 1 of ProDomain fatty acid desaturases. Conserved motifs and in silico analyses confirm these proteins are a Stearoyl-CoA '9 desaturase-1 with a preference for a 16-carbon substrate. The protein appears to have three structural domains and sustains no significant homology with previously established desaturase crystalline structures. Desaturases play important roles in lipid production and utilization important processes in leafhopper survival.