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United States Department of Agriculture

Agricultural Research Service

Research Project: GLASSY-WINGED SHARPSHOOTER AND PIERCE'S DISEASE Title: Molecular Analysis of Capsid Protein of Homalodisca Coagulata Virus-1. New Virus from Glassy-Winged Sharpshooter, Homalodisca Coagulata (Say) (Hemiptera: Cicadellidae)

Authors
item Katsar, Catherine
item Hunter, Wayne
item Chaparro, Jose - UNIVERSITY OF FLORIDA

Submitted to: Florida Entomological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 22, 2005
Publication Date: July 24, 2005
Citation: Katsar, C.S., Hunter, W.B., Chaparro, J.X. 2005. Molecular analysis of capsid protein of Homalodisca Coagulata Virus-1. New virus from Glassy-winged Sharpshooter, Homalodisca Coagulata (Say) (Hemiptera: Cicadellidae). 88th annual meeting of the Florida Entomological Society. July 24-27, 2005. Ft. Myers, FL. Paper No. DSP-12.

Technical Abstract: Capsid proteins have been shown to be a suitable target for phylogenetic studies in other insect viruses. A new insect infecting virus, isolated from the glassy-winged sharpshooter, GWSS, thus named, Homalodisca Coagulata Virus-1 (HoCV-1) demonstrated genetic relatedness to members within the Dicistroviridae, and appears to form a new clade within the newly recognized genus Cripavirus, “Cricket-Paralysis-like viruses”. Structural analysis of the HoCV-1 capsid protein indicated the presence of four domains with significant structural homology with previously established Cripavirus crystalline structures. HoCV-1 was found to increase sharpshooter mortality by ~20%, and was infective through microinjection, spraying, and bathing of insects with crude viral inoculum.

Last Modified: 12/21/2014
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