Technical Abstract: Listeria monocytogenes is a significant cause of food-borne disease and mortality, therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. Although conventional microarray subtyping analysis has been shown to be both high resolution and genetically informative, it is still relatively low throughput and technically challenging. Suspension microarray technology eliminates the technical issues associated with planar microarrays and allows high throughput subtyping of L. monocytogenes strains. In this study, a suspension array assay using dendrimer signal amplification allowed rapid and accurate serovar identification of L. monocytogenes strains using genomic DNA as target. The ability to subtype genomic DNA without PCR amplification allows probes to be designed to many different regions within the bacterial genome should allow high resolution subtyping not possible with multiplex PCR.