ARS | Publication request: DIFFERENTIAL GENE EXPRESSION OF ICTALURUS PUNCTATUS AFTER EDWARDSIELLA ICTALURI INFECTION

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: June 28, 2005
Publication Date: August 15, 2005
Citation: Yeh, H., Klesius, P.H. 2005. Differential gene expression of ictalurus punctatus after edwardsiella ictaluri infection. Genbank.

Technical Abstract: Channel catfish Ictalurus punctatus infected with Edwardsiella ictaluri results in $40-50 million annual losses in profits to catfish producers. To explore channel catfish gene expression after Edwardsiella ictaluri, a subtractive-suppression hybridization method was used. The result plasmid DNAs were sequenced from 5’-end using a T3 primer. Sequences were trimmed and aligned using Phred/Phrap/Consed programs. Trimmed sequences containing more than 100 bases were subject to BLAST against the GenBank databases. Top hits with a bit score >100 and an E-value <0.001 were selected for further annotation. In this report, 49 sequences from forward subtraction of infected channel catfish ovary cells (after 48 hr post infection) were identified.