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United States Department of Agriculture

Agricultural Research Service

Research Project: CONTROL AND PROTECTION TOOLS FOR INTEGRATED PEST MANAGEMENT OF MOSQUITOES AND FILTH FLIES Title: Population Genetic Structure of Anopheles Maculatus in Thailand

Authors
item Rongnoparut, Pornpimol - MAHIDOL UNIV.; THAILAND
item Rodpradit, Prinyada - MAHIDOL UNIV.; THAILAND
item Kongsawadworakul, Panida - MAHIDOL UNIV.; THAILAND
item Sithiprasasna, Ratana - USAMC-AFRIMS; THAILAND
item Linthicum, Kenneth

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 24, 2004
Publication Date: October 31, 2004
Citation: Rongnoparut, P., Rodpradit, P., Kongsawadworakul, P., Sithiprasasna, R., Linthicum, K. 2004. Population genetic structure of anopheles maculatus in thailand. 53rd Annual Meeting of the American Society of Tropical Medicine and Hygiene; Miami Beach, FL; November 7-11, 2004; 71(4):117.

Technical Abstract: Anopheles (Cellia) maculatus Theobald is major malaria vector in southern Thailand and peninsular Malaysia, and previous studies on the population genetics of this mosquito suggested that mountain ranges reduced gene flow among some populations. In this study we examine the genetic variance among 5 populations in southern Thailand by analyzing 7 microsatellite loci. All microsatellite loci exhibited moderated to high genetic variability for all populations with an average of 8.6 (range = 6.3-10.9) alleles per locus. Based on microsatellite analysis, geographic populations of southern populations of An. maculatus can be grouped in two clusters; one includes 3 populations located on the west side of the Phuket mountain range between 80 and 100 north latitude, and the other includes 2 populations east of the Phuket mountain range located between approximately 6.50 and 100 north latitude. Wright’s Fst and Slatkins’ Rst for all seven microsatellite loci indicated very low estimates of differentiation among the 3 populations west of the Phuket range (mean values of Fst and Rst = 0.0042 and 0.0031, respectively, corresponding to Nm values of 59.3 and 80.2, respectively), suggesting significant gene flow occurs among populations. However, there is some evidence that there is some restriction of gene flow between populations on different sides of the Phuket range (mean values of Fst and Rst = 0.0346 and 0.0606, respectively, corresponding to Nm values of 7.0 and 3.9, respectively). Populations separated by distances as little as 80 km exhibit restriction of gene flow when separated by geographic barriers, while populations separated by more than 650 km without geographic barriers exhibit greater gene flow.

Last Modified: 10/23/2014