ARS | Publication request: IDENTIFICATION AND CHROMOSOMAL LOCATION OF MAJOR GENES FOR RESISTANCE TO PYRENOPHORA TERES F. TERES AND P. TERES F. MACULATA IN BARLEY DOUBLED HAPLOID POPULATION.

Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 7, 2006
Publication Date: July 19, 2006
Citation: Friesen, T.L., Faris, J.D., Lai, Z., Steffenson, B.J. 2006. Identification and chromosomal location of major genes for resistance to Pyrenophora teres in a doubled-haploid barley population. Genome. 49:855-859

Interpretive Summary: Net blotch is one of the most economically important diseases of barley worldwide and has had impact on barley yield and quality in the United States due in part to the lack of genetic resistance available in commercial cultivars. Here, we used a barley doubled haploid population derived from the lines SM89010 and Q21861 to identify resistance genes effective against the seedling disease. A molecular map was used to identify chromosome locations of resistance genes. A gene for net blotch net type resistance was identified and this gene accounted for 89% of the resistance trait. A second gene for net blotch spot type resistance accounted for 64% of the disease variation. The markers closely linked to the resistance gene loci will be useful for marker-assisted selection.

Technical Abstract: Net blotch, caused by Pyrenophora teres f. teres and P. teres f. maculata, is one of the most economically important diseases of barley worldwide. Here, we used a barley doubled haploid population derived from the lines SM89010 and Q21861 to identify major genes and QTLs associated with seedling resistance to P. teres f. teres (net blotch net type) and P. teres f. maculata (net blotch spot type). A map consisting of simple sequence repeat and amplified fragment length polymorphic markers was used to identify chromosome locations of resistance loci. A single gene for net blotch net type resistance was located on the short arm of chromosome 6H, and this locus accounted for 89% of the phenotypic variation for resistance. A major QTL for net blotch spot type resistance was located on chromosome 4H and accounted for 64% of the disease variation. The markers closely linked to the resistance gene loci will be useful for marker-assisted selection.