ARS | Publication request: PATHOGENESIS OF MUCOR AND TRICHODERMA WERE COMPARED IN GOATS FOLLOWING INHALATION OF AEROSOLIZED STERILE FINE DUST

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: June 1, 2005
Publication Date: December 4, 2005
Citation: Purdy, C.W., Layton, R.C., Straus, D.C., Ayers, J. 2005. Pathogenesis of mucor and trichoderma were compared in goats following inhalation of aerosolized sterile fine dust [abstract]. In: Proceedings of Conference of Research Workers in Animal Diseases, 86th Annual Meeting, December 4-6, 2005, St. Louis, Missouri. Abstract Number A162.

Technical Abstract: A prospective randomized controlled study was conducted to compare the lung pathology induced by spores of Mucor ramosissimus and Trichoderma viride given by tracheal inoculation to non-immunosuppressed goats following exposure to a sterilized fine dust aerosol. There were 4 fungal treatment groups (two with dust and two without dust), a saline-tent control group, and a pen-control group. Five of the treatment groups were exposed to autoclaved feedyard fine (mean 7.72 (± 0.69SD) micro in diameter) dust for 4 hours in a specially constructed tent. Goats in the 4 treatment groups were then inoculated intratracheally with 30 mL of a fungal spore preparation. Saline-tent control goats were intratracheally inoculated with 30 ml of physiological saline solution following dust exposure, and the pen control goats were left untreated. The treatments were repeated weekly for 5 weeks. Mucor produced severe lung consolidation in all Mucor inoculated goats, and Trichoderma produced a softer lung consolidation in most Trichoderma inoculated goats. No lung consolidation lesions were observed in the saline-tent or pen controls. Both Mucor and Trichoderma spores induced lung fibrosis with formation of multiple granulomas with Langhan's type multinucleated giant cells; however, Mucor pathology was more severe and consistent. Endotoxin in the fine dust induced inflammation and appeared at first to cause more severe reaction to treatment, but no differences were observed later after multiple dust and fungal treatments.