Technical Abstract: Mint shoot tips were cryopreserved using a rapid cooling method with plant vitrification solution 2 (PVS2). PVS2 contains dimethyl sulfoxide, ethylene glycol, sucrose, and glycerol and dehydrates shoot tips to minimize the formation of intracellular ice during cooling to liquid nitrogen temperatures. We fixed and embedded shoot tips after each step of the cryopreservation procedure to quantify the extent of plasmolysis within the meristem, young leaf, older leaf, upper cortex, and lower cortex of shoot tips. The meristem cells were the smallest and least plasmolyzed and the large, older leaf and lower cortical cells were the most plasmolyzed. After culture, meristem cells were still intact up to seven days after warming while the older leaf and lower cortex cells appeared severely damaged by the cryopreservation process.