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United States Department of Agriculture

Agricultural Research Service

Title: Glucose Oxidase Effects on Wheat Flour Albumins and Gliadins

Author
item Tilley, Michael

Submitted to: Meeting Abstract
Publication Type: Other
Publication Acceptance Date: April 22, 2005
Publication Date: May 22, 2005
Citation: Tilley, M. 2005. Glucose oxidase effects on wheat flour albumins and gliadins. Program Book of the 3rd International Wheat Quality Conference. Meeting Abstract. p. 392

Technical Abstract: Chemical oxidants are routinely added to flour to modify rheological properties (shorten mixing time, improve gas retention, lower energy requirement for dough mixing) and enhance breadmaking performance (increase loaf volume and improve crumb structure). The elimination of potassium bromate, and possibly other chemical oxidant additives, presents a challenge to the baking industry. Alternative oxidation methods need to be found since industrial baking has been standardized with bromate. Substitution of chemical oxidants with enzymes is a desirable approach because enzymatic reactions are very specific, with little or no reactivity outside of the substrate. Oxidoreducing enzymes such as glucose oxidase (GOX) have been proposed as improvers for the baking industry. The mechanism of improvements caused by GOX is not understood. Following mixing wheat flour with and without the addition of GOX the different protein classes were extracted and analyzed by electrophoresis and size-exclusion HPLC. The most significant effects were observed to occur in the albumin (water-soluble) and gliadin (alcohol-soluble) protein groups. A significant increase in protein concentration and molecular weight distribution was observed in the albumin fraction by SE-HPLC. Further analysis revealed that this is due to changes in gliadin solubility. Gliadins are generally not soluble in water, however the inclusion of GOX in mixing renders the gliadins more water-soluble. The biochemical interactions responsible for this behavior and the possible effects on end-use properties are currently under investigation.

Last Modified: 10/25/2014
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