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United States Department of Agriculture

Agricultural Research Service

Title: A New Optical Immunosensor Platform for the Analysis of Pyrethroids and Ddt in Water

Authors
item Weber, Cristina - GSF-NATL RES CTR-GERMANY
item Forster, Stephan - GSF-NATL RES CTR-GERMANY
item Kremmer, Elisabeth - GSF-NATL RES CTR-GERMANY
item Rauch, Peter - CANDOR BIOSCIENCE-GERMANY
item Mulaa, Francis - U.OF NAIROBI, KENYA
item Stanker, Larry
item Kramer, Petra - GSF-NATL RES CTR-GERMANY

Submitted to: Pacifichem Symposium
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2005
Publication Date: June 1, 2005
Citation: Weber, C.M., Forster, S., Kremmer, E., Rauch, P., Mulaa, F.J., Stanker, L.H., Kramer, P.M. 2005. A new optical immunosensor platform for the analysis of pyrethroids and ddt in water. Program No. 303. 2005 Abstrac Viewer. Honolulu, Hawaii: International Chemical Congress of Pacific Basis Societies. Pacifichem Symposium.

Interpretive Summary: Contamination of water with pesticide residues can be found throughout the year. Especially in developing countries, there is an urgent need to provide cost-effective tools for on-site screening and control. For this purpose a new sensor (AQUA-OPTOSENSOR) using monoclonal antibodies (mAbs) was developed for two pesticides, pyrethroids and DDT. This sensor consists of an instrument, controlled by a laptop computer, and a single-use chip. It measures emission of fluorescent light and the amount of light emitted is inversely proportional to the pesticide concentration in the sample. Using this technique, it will be possible to monitor pollution in water matrices without cost-intensive laboratory analysis. Advantages and limitations of this technology will be discussed.

Technical Abstract: Contamination of water with pesticide residues occurs during application periods and runoffs. Some pesticide residues are also persistent and can be found throughout the year. Especially in developing countries, there is an urgent need to provide cost-effective tools for on-site screening and control. For this purpose a new optical immunosensor platform (AQUA-OPTOSENSOR) using monoclonal antibodies (mAbs) was established for the analysis of pyrethroids and DDT. This system is based on a formerly developed immunosensor and here consists of an instrument - controlled by a laptop - and a single-use chip. The pesticide coating antigen is adsorbed onto the surface of this chip. The anti-analyte mAb, which is labelled with a fluorophore (Oyster(R)-645:lambda ex 645 nm, lambda em 666 nm), is preincubated with the sample, and the mixture then is pumped over the surface of the chip (2 min). The fluorescence emission of the labelled antibody is then measured and the signal is inversely proportional to the analyte concentration. Standard curves for phenothrin and p,p'-DDT are shown. Both insecticides can be analysed in the low ppb level. Using this technique it will be possible to monitor pollution in water matrices without cost-intensive laboratory analysis. Advantages and limitations of this technology will be discussed.

Last Modified: 10/22/2014