ARS | Publication request: MOLECULAR MAPPING OF A RECESSIVE GENE IN BARLEY FOR RESISTANCE TO STRIPE RUST

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: March 1, 2005
Publication Date: June 1, 2005
Citation: Yan, G.P., Chen, X. 2005. Molecular mapping of a recessive gene in barley for resistance to stripe rust. National American Phytopathology Meetings. Phytopathology 95:S115.

Technical Abstract: Barley stripe rust, caused by Puccinia striiformis f. sp. hordei (PSH), is one of the most important barley diseases in the U.S. The disease is best controlled using resistant cultivars. Barley genotype Grannelose Zweizeilige (GZ) has a recessive gene (rpsGZ) that is effective against all PSH races identified so far in the U.S. To develop a molecular map for the gene, F8 recombinant inbred lines (RILs) were developed from the Steptoe X GZ cross. Seedlings of the parents and RILs were evaluated for resistance to races PSH-14 and PSH-54 under controlled greenhouse conditions. The resistance gene analog polymorphism (RGAP) technique was used to identify molecular markers for rpsGZ. A linkage map for the gene was constructed with 12 RGAP markers produced by the seven primer pairs. Two markers were completely co-segregating with the resistance locus, and another two markers were closely linked to the locus with a genetic distance of 0.9 and 2.0 cM, respectively. However, these four markers were present in the susceptible parent but not in the resistant parent. The closest marker that was present in the resistant parent but not in the susceptible parent had a genetic distance of 11.7 cM with the gene. Chromosomal-specific markers are being used to locate the gene on barley chromosomes.