|Goshroy, S - NEW MEXICO STATE UNIV.|
Submitted to: American Society of Sugarbeet Technologists
Publication Type: Proceedings
Publication Acceptance Date: March 7, 2005
Publication Date: August 1, 2005
Citation: Lartey, R.T., Caesar, T., Sol, N.I., Goshroy, S. 2005. Additional evidence of safflower (carthamus tinctorius) as an alternate host to cercospora beticola. In: Proceedings from the 33rd Biennial Meeting Agriculture. American Society of Sugarbeet Technologists, March 2-5, 2005, Palm Springs, California. p. 144-150. Technical Abstract: In the Northern Great Plains (NGP), safflower (Carthamus tinctorius L.) is increasingly being evaluated for rotation with sugarbeet (Beta vulgaris L). Safflower and sugarbeet are susceptible to two different species of Cercospora, Cercospora carthami and Cercospora beticola, respectively. This investigation of safflower as an alternate host of C. beticola was prompted by observation of unusual spot lesions on safflower in the NGP at Sidney, Montana. Previous report from our laboratory indicated that safflower has potential to serve as an alternate host of C. beticola. We present in this report additional evidence that safflower indeed is an alternate host of C. beticola. Safflower plants were infected with four C. beticola isolates (C1, C2, Sid1 and Sid2). Sugarbeets were inovulated single sport cultures of the four isolates from infected safflower lesions. Lesions of the symptoms were assayed by PCR for presence of C. beticola. Amplified PCR products were sequenced, imported into the Vector NTI (InforMax, Bethesda, MD) and aligned to compare with the C. beticola sequence from GenBank (Accession # AF443281). The aligned sequences from all four isolates from safflower and sugarbeet showed significant homology with sequence from C. beticola. Our results confirm the presence of C. beticola in lesions of infected safflower and substantiate safflower as a host of C. beticola.