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United States Department of Agriculture

Agricultural Research Service

Title: Hybsweeper: a Resource for Detecting High-Density Plate Coordinates

Submitted to: Biotechniques
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 14, 2005
Publication Date: September 1, 2005
Citation: Lazo, G.R., Lui, N., Gu, Y.Q., Kong, X., Coleman Derr, D., Anderson, O.D. 2005. Hybsweeper: a resource for detecting high-density plate coordinates. Biotechniques. 39:320-324.

Interpretive Summary: A software tool was developed to speed processing of information gained from DNA hybridization patterns stamped to high-density grids. The gridding patterns matched to clones which were housed in microtiter dishes. High-throughput methodologies have made it increasingly difficult to work with large archives of stored resources. The method developed to ease this effort was a software package called Hybsweeper, and was applied to laboratory work associated with simple probing methods, overgo probe hybridizations, and isolation of unique clones from saturation hybridization conditions.

Technical Abstract:

Last Modified: 4/22/2015