ARS | Publication request: AGROBACTERIUM-MEDIATED TRANSFORMATION OF SELECTED RED CLOVER GENOTYPES

Submitted to: Agrobacterium Protocols (2nd Edition)
Publication Type: Book / Chapter
Publication Acceptance Date: February 14, 2005
Publication Date: May 4, 2006
Citation: Sullivan, M.L., Quesenberry, K.H. 2006. Red Clover (Trifolium pratense). In: Wang, K. (editor). Methods in Molecular Biology: Agrobacterium Protocols. 2nd edition. Totowa, NJ: Humana Press. Vol. 343, p. 369-384.

Technical Abstract: Genetic modification of plants by the insertion of transgenes can be a powerful experimental approach to answer basic questions about gene product function, and also can be used to make improved crop varieties for use in the field. To apply this powerful tool to red clover, an important forage legume, a population of red clover with high potential for regeneration in tissue culture has been developed. Here we provide a detailed procedure for Agrobacterium-mediated transformation of genotypes derived from this regenerable population. We have successfully used this methodology to over-express a b-glucuronidase reporter gene and silence an endogenous polyphenol oxidase gene in red clover.