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United States Department of Agriculture

Agricultural Research Service

Title: Morphological and Molecular Characterization of Heterodera Sacchari Luc & Merny, 1963, and H. Goldeni Handoo & Ibrahim, 2002 (Nematoda: Heteroderidae)

item Maafi, Zahra - PPDR INSTITUTE, IRAN
item Sturhan, Dieter - NEMATOL INST, GERMANY
item Handoo, Zafar
item Moens, Maurice - ARC, MERELBEKE, BELGIUM
item Subbotin, Sergei - UNIV CA, RIVERSIDE

Submitted to: Russian Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2005
Publication Date: July 1, 2005
Citation: Maafi, Z.T., Sturhan, D., Handoo, Z.A., Mor, M., Moens, M., Subbotin, S.A. 2005. Morphological and molecular characterization of heterodera sacchari luc & merny, 1963, and h. goldeni handoo & ibrahim, 2002 (nematoda: heteroderidae). Russian Journal of Nematology. 13(2):152-153.

Technical Abstract: Heterodera sacchari and H. goldeni belong to the Sacchari group and share several diagnostic characters: a strongly developed underbridge with finger-like projections in the cyst, prominent anchor-shaped stylet knobs, and three incisures in the lateral field in second-stage juveniles. Heterodera sacchari parasitizing sugarcane and rice was found from several African and Asian countries. Heterodera goldeni attacking Qasabagrass (Panicum coloratum) was recently described from Egypt. Comparative morphological and molecular analyses of a previously reported cyst nematode species parasitizing Phragmites sp. and Juncus acutus from Iran and an unidentified cyst nematode parasitizing Kikuyu grass (Pennisetum clandestinum) from Israel revealed that these nematodes also belong to H. goldeni. Morphological and morphometrical characters for cysts and second-stage juveniles for differentiation of H. goldeni from H. sacchari and H. leuceilyma are provided and discussed. Heterodera sacchari has generally smaller cysts than H. goldeni (average 640-735 x 430-470 micrometers vs 707-766 x 437-481 micrometers) and shorter fenestral length (average 40-52 micrometers vs 60-61 micrometers). Second-stage juveniles of Heterodera goldeni differ from those of H. sacchari by having slightly longer tail (average 59-65 vs range 50-62 micrometers) and longer hyaline part of tail (average 34-38 vs 26-32 micrometers). The phasmid in H. goldeni is situated much closer to the anus. The ITS-rRNA sequences of H. sacchari and H. goldeni differed from each other by 6 insertion/deletion events and 22 nucleotides. The ITS sequences of H. sacchari from Ghana and Cote d’Ivoire were almost identical, while difference between ITS sequences for H. goldeni samples varied from 1 to 6 nucleotides (0.1-0.5%). Phylogenetic relationships between species and populations of the Sacchari group based on the ITS-rRNA sequences are presented.

Last Modified: 4/22/2015
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