|Leeb, Tosso - INST ANIM BRDG, GERMANY|
Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 17, 2005
Publication Date: July 1, 2005
Citation: Leeb, T., Rohrer, G.A. 2005. Brief note. characterization and linkage mapping of four gene-associated porcine microsatellites. Animal Genetics. 36:279-280. Interpretive Summary: Two important genes necessary for everyday function of cells and two genes important for semen production were mapped in the pig genome. The genes mapped were CDC37 (cell division cycle 37 homolog), PRSS11 (serine protease 11), PSP-I (porcine seminal plasma protein I) and PSP-II (porcine seminal plasma protein II). All genes were mapped by three independent methods. As expected, CDC37 mapped to pig chromosome 2 and the other three genes mapped to pig chromosome 14. CDC37 maps where an important association for pork tenderness resides and this assignment helps to define the similarities of this region in the pig with the human genome. The genes PSP-I and PSP-II are potential candidate genes for male fertility traits in pigs.
Technical Abstract: Porcine BAC and one PAC clones containing CDC37 (cell division cycle 37 homolog), PRSS11 (serine protease 11), PSP-I (porcine seminal plasma protein I) and PSP-II (porcine seminal plasma protein II) genes were sequenced. Microsatellite repetitive elements were identified in each clone. Primer pairs were designed to amplify each of the microsatellites and to map each gene. Microsatellites were mapped in the MARC Swine Reference Population. In addition, BAC and PAC clones were physically mapped by FISH and STS markers from these clones were mapped on the IMpRH panel. As expected, CDC37 mapped to SSC2q1.3-2.1 on the physical map and to position 61.6 cM on the MARC swine linkage map at the same location as SWR468 and SWR1342. The other three genes were physically assigned to SSC14q2.8-2.9. Their best order (not statistically significant from other permutations) on SSC14 is SW1557 (81.8 cM) - PRSS11 (83.7 cM) - PSP2 (86.5 cM) - PSP1 (86.5 cM) - SW2515 (103.0 cM). The order of these three genes is consistent with both the RH mapping results as well as their order in the human genome and their location concurs with the physical assignments.