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United States Department of Agriculture

Agricultural Research Service

Title: Isolation and Characterization of Eight Microsatellite Loci from Phaedranassa Tunguraguae (Amaryllidaceae)

item Meerow, Alan
item Francisco-Ortega, Javier - FLORIDA INTERNATIONAL UNI

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 28, 2005
Publication Date: January 27, 2006
Citation: Oleas, N.H., Meerow, A.W., Francisco-Ortega, J. 2006. Isolation and characterization of eight microsatellite loci from Phaedranassa tunguraguae (Amaryllidaceae). Molecular Ecology Notes. 5:791-793

Interpretive Summary: We have isolated eight genetic markers from the DNA of Phaedranassa tunguraguae, an endangered species of ornamental flower bulb native to Ecuador. These highly variable markers allow the investigations of patterns of genetic variation in this species, which will be useful for conservation efforts as well as improvement of the species for floriculture.

Technical Abstract: Phaedranassa tunguraguae is an endangered species endemic to Ecuador. Eight highly polymorphic microsatellite loci were isolated from an enriched genomic library for this species. Levels of polymorphism were evaluated using a total of 31 individuals from a single natural population. An average of 14.1 alleles per locus was detected, and observed heterozygosity ranged from 0.387 to 0.903. All but one locus depart significantly from Hardy-Weinberg Equilibrium. These loci are the first microsatellite primers isolated for Amaryllidaceae and will be utilized to investigate patterns of genetic variation of P. tunguraguae, which will contribute data relevant to the conservation of the species.

Last Modified: 4/19/2015
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